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dtaf donkey anti rabbit igg  (Jackson Immuno)


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    Jackson Immuno dtaf donkey anti rabbit igg
    Mouse skin cryosections were incubated with control rabbit <t>IgG</t> (a, d), anti-mCOL7 IgG (b, e) or EndoS-treated rabbit anti-mCOL7 IgG (c, f) at the concentrations indicated. Bound antibody was visualized by fluorescence microscopy using a <t>DTAF-conjugated</t> secondary antibody and fluorescence intensity was quantified using Image J software (g). Data of one representative experiment (a–f) are shown or given as mean ± s.d. of 8 independent experiments. Increasing concentrations of control rabbit IgG, rabbit anti-mCOL7 IgG or EndoS-treated rabbit anti-mCOL7 IgG were exposed to immobilized mCOL7 (h) and bound IgG was detected by a secondary antibody. Data are presented as mean ± s.d. n = 3, *indicates statistically significant differences ( p <0.05) between EndoS-treated and untreated IgG.
    Dtaf Donkey Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 10764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/donkey+anti+rabbit+dtaf/Donkey+Anti-Rabbit+IgG/pmc03913582-31-27-29
    Average 96 stars, based on 10764 article reviews
    dtaf donkey anti rabbit igg - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "EndoS Reduces the Pathogenicity of Anti-mCOL7 IgG through Reduced Binding of Immune Complexes to Neutrophils"

    Article Title: EndoS Reduces the Pathogenicity of Anti-mCOL7 IgG through Reduced Binding of Immune Complexes to Neutrophils

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0085317

    Mouse skin cryosections were incubated with control rabbit IgG (a, d), anti-mCOL7 IgG (b, e) or EndoS-treated rabbit anti-mCOL7 IgG (c, f) at the concentrations indicated. Bound antibody was visualized by fluorescence microscopy using a DTAF-conjugated secondary antibody and fluorescence intensity was quantified using Image J software (g). Data of one representative experiment (a–f) are shown or given as mean ± s.d. of 8 independent experiments. Increasing concentrations of control rabbit IgG, rabbit anti-mCOL7 IgG or EndoS-treated rabbit anti-mCOL7 IgG were exposed to immobilized mCOL7 (h) and bound IgG was detected by a secondary antibody. Data are presented as mean ± s.d. n = 3, *indicates statistically significant differences ( p <0.05) between EndoS-treated and untreated IgG.
    Figure Legend Snippet: Mouse skin cryosections were incubated with control rabbit IgG (a, d), anti-mCOL7 IgG (b, e) or EndoS-treated rabbit anti-mCOL7 IgG (c, f) at the concentrations indicated. Bound antibody was visualized by fluorescence microscopy using a DTAF-conjugated secondary antibody and fluorescence intensity was quantified using Image J software (g). Data of one representative experiment (a–f) are shown or given as mean ± s.d. of 8 independent experiments. Increasing concentrations of control rabbit IgG, rabbit anti-mCOL7 IgG or EndoS-treated rabbit anti-mCOL7 IgG were exposed to immobilized mCOL7 (h) and bound IgG was detected by a secondary antibody. Data are presented as mean ± s.d. n = 3, *indicates statistically significant differences ( p <0.05) between EndoS-treated and untreated IgG.

    Techniques Used: Incubation, Fluorescence, Microscopy, Software

    Related Articles

    other:

    Article Title: TAL1/SCL is expressed in endothelial progenitor cells/angioblasts and defines a dorsal-to-ventral gradient of vasculogenesis.
    Article Snippet: Secondary antibodies used were combinations of donkey anti-rabbit Cy5, donkey anti-mouse DTAF, donkey anti-rabbit DTAF, or donkey anti-mouse Cy5 (Jackson Immunoresearch Labo-Preparation of Anti-TAL1 Antibody ratories, Inc., West Grove, PA).

    Article Title: Expression of matrix-metalloproteinases and their inhibitors in human cholesteatomas.
    Article Snippet: Expression of Matrix-Metalloproteinases and their Inhibitors in Human Cholesteatomas Matthias Schönermark, Birte Mester, Hans-Georg Kempf, Jörg Bläser, Harald Tschesche & Thomas Lenarz To cite this article: Matthias Schönermark, Birte Mester, Hans-Georg Kempf, Jörg Bläser, Harald Tschesche & Thomas Lenarz (1996) Expression of Matrix-Metalloproteinases and their Inhibitors in Human Cholesteatomas, Acta Oto-Laryngologica, 116:3, 451-456, DOI: 10.3109/00016489609137872 To link to this article: http://dx.doi.org/10.3109/00016489609137872



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    Image Search Results


    Mouse skin cryosections were incubated with control rabbit IgG (a, d), anti-mCOL7 IgG (b, e) or EndoS-treated rabbit anti-mCOL7 IgG (c, f) at the concentrations indicated. Bound antibody was visualized by fluorescence microscopy using a DTAF-conjugated secondary antibody and fluorescence intensity was quantified using Image J software (g). Data of one representative experiment (a–f) are shown or given as mean ± s.d. of 8 independent experiments. Increasing concentrations of control rabbit IgG, rabbit anti-mCOL7 IgG or EndoS-treated rabbit anti-mCOL7 IgG were exposed to immobilized mCOL7 (h) and bound IgG was detected by a secondary antibody. Data are presented as mean ± s.d. n = 3, *indicates statistically significant differences ( p <0.05) between EndoS-treated and untreated IgG.

    Journal: PLoS ONE

    Article Title: EndoS Reduces the Pathogenicity of Anti-mCOL7 IgG through Reduced Binding of Immune Complexes to Neutrophils

    doi: 10.1371/journal.pone.0085317

    Figure Lengend Snippet: Mouse skin cryosections were incubated with control rabbit IgG (a, d), anti-mCOL7 IgG (b, e) or EndoS-treated rabbit anti-mCOL7 IgG (c, f) at the concentrations indicated. Bound antibody was visualized by fluorescence microscopy using a DTAF-conjugated secondary antibody and fluorescence intensity was quantified using Image J software (g). Data of one representative experiment (a–f) are shown or given as mean ± s.d. of 8 independent experiments. Increasing concentrations of control rabbit IgG, rabbit anti-mCOL7 IgG or EndoS-treated rabbit anti-mCOL7 IgG were exposed to immobilized mCOL7 (h) and bound IgG was detected by a secondary antibody. Data are presented as mean ± s.d. n = 3, *indicates statistically significant differences ( p <0.05) between EndoS-treated and untreated IgG.

    Article Snippet: The capacity of rabbit anti-mCOL7 to bind its antigen was tested by indirect immunofluorescence (IF) staining of sections (6 µm) derived from healthy C57BL/6 mouse skin using DTAF-donkey-anti-rabbit IgG (Jackson Immunoresearch Laboratory, West Grove, PA, USA) as detection antibody.

    Techniques: Incubation, Fluorescence, Microscopy, Software